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memglowtm 640  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc memglowtm 640
    Memglowtm 640, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/memglowtm+640/MemGlow+640+Fluorogenic+Membrane+Probe/bio_rxiv__64898__2026__04__16__718987-76-7-9
    Average 95 stars, based on 31 article reviews
    memglowtm 640 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Labeling:

    Article Title: Magnetically Labelled iPSC‐Derived Extracellular Vesicles Enable MRI/MPI‐Guided Regenerative Therapy for Myocardial Infarction
    Article Snippet: For HUVEC-based assays, wells were pre-coated with 10 μg/mL human fibronectin (Sigma, F1141) in phosphate-buffered saline (PBS) for 30 minutes at 37°C prior to cell seeding. .. EV Labeling and Quantification iPSC-derived EVs were fluorescently labeled using MemGlowTM 640 (Cytoskeleton, MG04) by incubating with 1 μM dye for 1 hour at room temperature in darkness. .. Unbound dye was removed using Vesi-SEC-micro size-exclusion columns (Vesiculab).

    Article Title: A novel reporter mouse for astrocyte-derived extracellular vesicles reveals trafficking of cargo to neuronal mitochondria
    Article Snippet: For live-cell imaging, mitochondria were labeled with 500 nM MitoTrackerTM Red CM-H2XRos (Invitrogen) for 45 min at 37 °C, followed by washing with phenol red-free, serum-free medium to remove excess dye. .. Plasma membranes were labeled with 100 nM MemGlowTM 640 (Cytoskeleton Inc.) prepared in the same medium, incubated for 5 min at 37 °C, and then washed. .. The glass-bottom plates were mounted on a Nikon CSU-W1 SoRa spinning-disk confocal microscope.

    Incubation:

    Article Title: Development and Characterization of a Novel α-Synuclein-PEST H4 Cell Line for Enhanced Drug Screening in α-Synucleinopathies
    Article Snippet: Following three PBS wash steps, cells were incubated with a secondary antibody solution containing 1:1000 secondary antibody (goat anti-mouse AF555, Cat# A32727, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with DAPI (1:1000 dilution) for 1 h at room temperature. .. Following two PBS wash steps, cells were incubated with a 1:1000 dilution of MemGlowTM 640 (Cat# MG04, Cytoskeleton, Denver, CO, USA) for 15 min and washed twice in PBS prior to imaging. .. The cells were then imaged in PBS on a Perkin-Elmer Operetta CLS microscope system and analyzed in Harmony ® 4.9.

    Article Title: Development and Characterization of a Novel α-Synuclein-PEST H4 Cell Line for Enhanced Drug Screening in α-Synucleinopathies.
    Article Snippet: Following three PBS wash steps, cells were incubated with a secondary antibody solution containing 1:1000 secondary antibody (goat anti-mouse AF555, Cat# A32727, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with DAPI (1:1000 dilution) for 1 h at room temperature. .. Following two PBS wash steps, cells were incubated with a 1:1000 dilution of MemGlowTM 640 (Cat# MG04, Cytoskeleton, Denver, CO, USA) for 15 min and washed twice in PBS prior to imaging. .. The cells were then imaged in PBS on a Perkin-Elmer Operetta CLS microscope system and analyzed in Harmony® 4.9.

    Article Title: A novel reporter mouse for astrocyte-derived extracellular vesicles reveals trafficking of cargo to neuronal mitochondria
    Article Snippet: For live-cell imaging, mitochondria were labeled with 500 nM MitoTrackerTM Red CM-H2XRos (Invitrogen) for 45 min at 37 °C, followed by washing with phenol red-free, serum-free medium to remove excess dye. .. Plasma membranes were labeled with 100 nM MemGlowTM 640 (Cytoskeleton Inc.) prepared in the same medium, incubated for 5 min at 37 °C, and then washed. .. The glass-bottom plates were mounted on a Nikon CSU-W1 SoRa spinning-disk confocal microscope.

    Imaging:

    Article Title: Development and Characterization of a Novel α-Synuclein-PEST H4 Cell Line for Enhanced Drug Screening in α-Synucleinopathies
    Article Snippet: Following three PBS wash steps, cells were incubated with a secondary antibody solution containing 1:1000 secondary antibody (goat anti-mouse AF555, Cat# A32727, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with DAPI (1:1000 dilution) for 1 h at room temperature. .. Following two PBS wash steps, cells were incubated with a 1:1000 dilution of MemGlowTM 640 (Cat# MG04, Cytoskeleton, Denver, CO, USA) for 15 min and washed twice in PBS prior to imaging. .. The cells were then imaged in PBS on a Perkin-Elmer Operetta CLS microscope system and analyzed in Harmony ® 4.9.

    Article Title: Development and Characterization of a Novel α-Synuclein-PEST H4 Cell Line for Enhanced Drug Screening in α-Synucleinopathies.
    Article Snippet: Following three PBS wash steps, cells were incubated with a secondary antibody solution containing 1:1000 secondary antibody (goat anti-mouse AF555, Cat# A32727, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with DAPI (1:1000 dilution) for 1 h at room temperature. .. Following two PBS wash steps, cells were incubated with a 1:1000 dilution of MemGlowTM 640 (Cat# MG04, Cytoskeleton, Denver, CO, USA) for 15 min and washed twice in PBS prior to imaging. .. The cells were then imaged in PBS on a Perkin-Elmer Operetta CLS microscope system and analyzed in Harmony® 4.9.

    Clinical Proteomics:

    Article Title: A novel reporter mouse for astrocyte-derived extracellular vesicles reveals trafficking of cargo to neuronal mitochondria
    Article Snippet: For live-cell imaging, mitochondria were labeled with 500 nM MitoTrackerTM Red CM-H2XRos (Invitrogen) for 45 min at 37 °C, followed by washing with phenol red-free, serum-free medium to remove excess dye. .. Plasma membranes were labeled with 100 nM MemGlowTM 640 (Cytoskeleton Inc.) prepared in the same medium, incubated for 5 min at 37 °C, and then washed. .. The glass-bottom plates were mounted on a Nikon CSU-W1 SoRa spinning-disk confocal microscope.



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